Al-Kunooze Scientific Journal

Protective effects of broccoli sprout extract on Caspase 3 and liver function in rat female treated with methotrexate

Document Type : Original Article

Author

Nuha Hussam Abdulwahab

جامعة تكريت

https://doi.org/10.36582/ksj.2026.169017.1024
Abstract
Methotrexate (MTX) is an analog of folic acid utilized as a low-dose treatment for autoimmune diseases, such as PsA or RA and a high-dose treatment for cancer. MTX increased the apoptosis by switching on caspase 3, which is switching on death protease, stimulating the specific incision of cellular proteinase. We chose BSE for this research because it content from glucoraphanin higher than adult broccoli, This research aimed to determine the levels of casp -3 and liver function to determinated protective effects of BSE in mitigating MTX impact in rat female due to lack of studies that addressed this matter especially in females which spread among them lots of autoimmune diseases requires MTX use. RT-qPCR technique was used to determination levels of gene expression subsequent to RNA extraction. Our study suggested that BSE enhance liver functions and caspase 3 expression in rat that treated with MTX and broccoli sprout extract and in groups treated with BSE only if we compared it with control and MTX groups.

Keywords

Methotrexate(MTX)
Broccli sproute extract (BSE)
cysteine-containing aspartic acid-specific proteases) CASP3 Alanine aminotranseferase(ALT)
Aspartate aminotraneferase(AST)
Alkaline phosphatase (ALP
Crossmark
Al-Kunooze Scientific Journal
Volume 12, Issue 2
Spring 2026
Pages 66-76

  • Receive Date 27 January 2026
  • Revise Date 12 February 2026
  • Accept Date 13 February 2026
  • First Publish Date 13 February 2026
  • Publish Date 01 April 2026

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