Male infertility exists as a complex medical issue which depends on multiple physiological and
biochemical factors particularly lipid metabolism and genetic integrity. The research evaluated how
lipid profile characteristics relate to DNA fragmentation index (DFI) in Iraqi men who have either
normal or abnormal semen test results. The study enrolled 90 male participants who included 46 men
with normal semen characteristics between ages 20 to 45 and 44 men with abnormal semen
parameters. Semen samples underwent analysis for standard parameters and lipid profile and DFI.
The aberrant semen group displayed elevated triglyceride (TG) levels at 19.32±5.60 mg/dl which
exceeded normal group levels at 14.50±4.62 mg/dl (P<0.01). The analysis revealed no significant
variation between cholesterol (CHO) levels (P=0.410) but LDL levels of the aberrant semen group
(6.38±4.12 mg/dl) significantly diverged from the normal group levels (9.03±3.43 mg/dl with
P<0.01). The DNA fragmentation index (DFI) levels of the aberrant semen group exceeded those of
the normal semen group by a significant margin (31.66±16.15% versus 13.54±9.49%, P<0.01). This
indicates that men with poor semen quality typically present with sperm DNA damage. The research
findings suggest that abnormal lipid metabolism together with increased DNA fragmentation plays a
role in male infertility development particularly among men with abnormal semen analysis results.