Background: needle stick injuries expose healthcare workers to blood-borne viruses including HIV, hepatitis B, and hepatitis C, they pose a serious occupational danger. To lessen these risks, infection prevention procedures must be understood and followed. Objective: This research seeks to assess healthcare workers' understanding, habits, and views of needle stick injuries and to determine the variables affecting their compliance with preventative measures. Methods: descriptive cross-sectional research study with 400 healthcare workers from various health facilities. We gathered data using standardized questionnaires that focused on demographic factors, functional roles, awareness, practices, and NSI views. Result: The majority of participants were aged 20–29 years (62.5%), mostly male (70.8%), and resided in metropolitan regions (75.8%). Moderate awareness levels were seen in 54.8% of healthcare workers, accompanied by notable deficiencies in knowledge of viral survival periods. Good behaviors were noted in 59.2% of participants, especially for infection control measures such as hand cleanliness and sharps disposal. Conclusion: The study found that most healthcare workers have a moderate level of awareness about needle stick injuries.