Objective: The Disease Control and Prevention Centers points out that the number of deaths from antibiotic resistance nearly doubles every year in countries around the world, so it’s imperative to spread and increase knowledge among patients and health providers about how to prevent the misuse of antibiotics and the importance of taking them as properly prescribed by a physician. Methods: A study was conducted on a sample of 200 students at the colleges of nursing and Al Zahra medicine to assess their knowledge of antibiotic misuse and the resulting resistance in pathogenic bacteria by exposing them to a questionnaire questions, the results were collected and analyzed statistically. Results: it has been showed a ratio of %51.5 of all students had a good level of knowledge, while %16.5 of them are poorly informed and there were no significant differences between nursing and medical students in knowledge where P˃0.05. Also, a significant difference was found between the levels of answers to questions for part 3 higher than for part 2 of the questionnaire (P˂0.05). The correct answer rate per student was 7 versus 3 wrong for each part of the questionnaire. Conclusion: More than half the students (%51.5) had a good level of knowledge, (%32) were average, and the rest (%16.5) were poor, so these weak students need further education about the benefits and dangers of antibiotics and their proper use.