About one-third of humans have the bacterium S. aureus, recognized for its opportunistic tendency to invade the anterior nares. S. aureus and/or (MRSA) can colonize the anterior nares and go on to colonize the skin and other anatomical areas. As such, these pathogens have the capacity to cause a wide variety of endogenous diseases. To examine the frequency of nasal carriage of S. aureus or MRSA among some diabetic patients in Iraq/Baghdad, nasal swabs were collected from 50 Type 2 diabetic patients and 50 control subjects. 50 blood samples were collected (patients with T2DM) collected from different age groups and the duration of the disease in patients who attended “The Specialist Center for Diseases of Endocrine and Diabetes” in Baghdad. Another 50 blood samples were collected from normal healthy controls at different ages and genders. The period of study was from February 2021 and April 2022. The results showed that there were highly significant differences between T2DM patients and healthy controls in FBG and HbA1c at (P≤0.01). A nasal swab was obtained to detect colonization by S. aureus. A repeat swab was obtained from all patients with type 2 diabetes and healthy controls to estimate persistent S. aureus carriage. According to the morphology of the cultures and biochemical characteristics, API staph was used to confirm the diagnosis. and molecular detection using nuc gene, the S. aureus isolates were identified MRSA isolates were identified by PCR using a specific primer of mecA The prevalence of S. aureus nasal colonization was 52% and of the persistent carriage 30%. From all patients with Type 2 diabetes, and The prevalence of S. aureus nasal colonization was 32% and of the persistent carriage 12% in control healthy. with 24% of the total patients being colonized specifically with MRSA.